Type

Journal Article
Abstract

Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of intensity-based glutamate-sensing fluorescent reporter (iGluSnFR) that are functionally brighter; detect submicromolar to millimolar amounts of glutamate; and have blue, cyan, green, or yellow emission profiles. These variants could be imaged in vivo in cases where original iGluSnFR was too dim, resolved glutamate transients in dendritic spines and axonal boutons, and allowed imaging at kilohertz rates.

Journal
Nature methods
Volume
15
Issue
11
Pages
936-939
Date Published
12/2018
ISSN Number
1548-7105
Alternate Journal
Nat Methods
PMID
30377363