@article{189056,
  author = {Jonathan Marvin and Benjamin Scholl and Daniel Wilson and Kaspar Podgorski and Abbas Kazemipour and Johannes M{\"u}ller and Susanne Schoch and Francisco Quiroz and Nelson Rebola and Huan Bao and Justin Little and Ariana Tkachuk and Edward Cai and Adam Hantman and Samuel S.-H. Wang and Victor DePiero and Bart Borghuis and Edwin Chapman and Dirk Dietrich and David DiGregorio and David Fitzpatrick and Loren Looger},
  title = {Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR.},
  abstract = {<p>Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of intensity-based glutamate-sensing fluorescent reporter (iGluSnFR) that are functionally brighter; detect submicromolar to millimolar amounts of glutamate; and have blue, cyan, green, or yellow emission profiles. These variants could be imaged in vivo in cases where original iGluSnFR was too dim, resolved glutamate transients in dendritic spines and axonal boutons, and allowed imaging at kilohertz rates.</p>
},
  year = {2018},
  journal = {Nature methods},
  volume = {15},
  pages = {936-939},
  month = {12/2018},
  issn = {1548-7105},
  doi = {10.1038/s41592-018-0171-3},
  language = {eng},
}