@article{189116,
  author = {Yan Zhang and M{\'a}rton R{\'o}zsa and Yajie Liang and Daniel Bushey and Ziqiang Wei and Jihong Zheng and Daniel Reep and Gerard Broussard and Arthur Tsang and Getahun Tsegaye and Sujatha Narayan and Christopher Obara and Jing-Xuan Lim and Ronak Patel and Rongwei Zhang and Misha Ahrens and Glenn Turner and Samuel S.-H. Wang and Wyatt Korff and Eric Schreiter and Karel Svoboda and Jeremy Hasseman and Ilya Kolb and Loren Looger},
  title = {Fast and sensitive GCaMP calcium indicators for imaging neural populations},
  abstract = {<p>Calcium imaging with protein-based indicators is widely used to follow neural activity in intact nervous systems. The popular GCaMP indicators are based on the calcium-binding protein calmodulin and the RS20 peptide. These sensors report neural activity at timescales much slower than electrical signaling, limited by their biophysical properties and trade-offs between sensitivity and speed. We used large-scale screening and structure-guided mutagenesis to develop and optimize several fast and sensitive GCaMP-type indicators. The resulting {\textquoteleft}jGCaMP8{\textquoteright} sensors, based on calmodulin and a fragment of endothelial nitric oxide synthase, have ultra-fast kinetics (rise times, 2 ms) and still feature the highest sensitivity for neural activity reported for any protein-based sensor. jGCaMP8 sensors will allow tracking of larger populations of neurons on timescales relevant to neural computation.</p>
},
  year = {2021},
  journal = {bioRxiv},
  month = {11/2021},
  doi = {10.1101/2021.11.08.467793},
}